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Wim van Egmond
Joined: 08 Apr 2005 Posts: 440 Location: Rotterdam, the Netherlands
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Posted: Wed May 11, 2005 4:47 am Post subject: first flash result, a synchaeta with fungi |
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I finally post an image made with the new flash on the microscope. The first moving organism I photographed was this Synchaeta, a predatory rotifer. I had it trapped under the coverslip so I could have done this wihout flash. But I think it is sharper than the images I made without flash. And the little cilia are also sharp.
I was intrigued by this type of fungi that spead throughout this predatory rotifer. There is also an enigmatic round object left to the big trophi. Does anybody have a suggestion what it may be?
This image was made with a 25X plan apo I recently bought from a friend. I am happy with it. The depth of field is very shallow especially with the full aperture used in DIC but I combined two focus layers to get the most important parts in focus.
I hope to have time to do more photography soon. I hope to do dark field too!
Wim |
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twebster Site Admin

Joined: 19 Apr 2004 Posts: 1518 Location: Phoenix "Valley of the Sun", Arizona, USA
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Posted: Wed May 11, 2005 8:01 am Post subject: |
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WOW, Wim
Your DIC images just knock my socks off About the time I think I'm catching up to some of you image-wise, you go and raise the bar another notch What a beautiful and intriguing image. I'm just fascinated by how well the internal structures are revealed in DIC. I don't know what that bright, round object is, though.
It's amazing how infested with fungi and parasites rotifers can become, yet they can still function somewhat. Humans are wimps in comparison. If we suffered an infestation as bad as this rotifer, we would have succumbed to it much sooner.
Where do you find rotifers, like this one? Do these rotifers live in colder waters? All I can find in the ponds around me are bdelloid rotifers and those little, rotund loricate rotifers. Our ponds get pretty warm here in Phoenix, Arizona, USA, and I just never see rotifers like this one or like the ones Gerd has posted.
Best regards as always, my friend.  _________________ Tom Webster
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Phoenix "The Valley of the Sun", Arizona, USA
Think about this...maybe Murphy is an optimist!!! |
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piotr

Joined: 28 Apr 2004 Posts: 445 Location: San Diego
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Posted: Wed May 11, 2005 10:16 am Post subject: |
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Amazing!!!
Very interesting subject. Details and resolution are incredible. What is the red structure next to a tail?
You pushed optical microscopy to its limits, Wim. The planapo objective + flash + DIC (+Wim ) is the best combination I can imagine. _________________ Piotr |
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Oliver Skibbe
Joined: 28 Apr 2005 Posts: 19 Location: Germany
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Posted: Wed May 11, 2005 2:30 pm Post subject: |
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Hi, Wim!
I have never seen anything like this before, but it sure doesn't look like a fungal parasite. There are no hyphae visible inside the rotifer.
Maybe sporozoan infection? _________________ Oliver
www.larger-than-life.de |
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GreenLarry

Joined: 07 May 2005 Posts: 360 Location: North East of England
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Posted: Thu May 12, 2005 7:13 am Post subject: |
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Fantastic image Wim, the fungi are those lozenge shaped objects I take it?
The detail here is amazing, what flash set up do you use, is it a special one for photomicroscopy? |
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twebster Site Admin

Joined: 19 Apr 2004 Posts: 1518 Location: Phoenix "Valley of the Sun", Arizona, USA
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Posted: Wed May 18, 2005 6:43 am Post subject: |
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Hi ya' Wim
I keep returning to view this stunning image. I can't t imagine how the rotifer still functions
Best regards as always, my friend  _________________ Tom Webster
Administrator
Phoenix "The Valley of the Sun", Arizona, USA
Think about this...maybe Murphy is an optimist!!! |
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nzmacro Site Admin

Joined: 20 Apr 2004 Posts: 1604 Location: New Zealand
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Posted: Fri May 20, 2005 9:06 pm Post subject: |
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Totally incredible work. The details are amazing. Congrats Wim, excellent work and shot.
Danny. |
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