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moth brain stitch

 
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Steve West



Joined: 10 Oct 2004
Posts: 545
Location: Tucson, AZ USA

PostPosted: Thu Dec 09, 2004 6:53 pm    Post subject: moth brain stitch Reply with quote

Here's an image of the whole moth brain section. I stitched 35 200x frames together to make this (using ImageAssembler). The resulting file is about 30MB. I printed a 3-foot long panorama from the stitch which shows tons of detail (mostly of the air tubes). Unfortunately, by the time I had time to take this picture sequence, most of the stain had bled out of the brain and into the glycerine. Too bad. Again, this is brightfield with a 20x phaco.



The brain is about 4mm across. The two large outer structures are the optic lobes, and we already talked about the antennae lobes. The U-shaped opening at the center is the esophagus! The billions of black fibers are air tubes supplying the oxygen.

Steve
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Frez



Joined: 08 May 2004
Posts: 419
Location: New Hampshire USA

PostPosted: Thu Dec 09, 2004 7:02 pm    Post subject: Reply with quote

That's incredible! It sounds like a lot of sewing. Smile
Being mostly familiar with mammalian brains, this sure looks odd or primitive. Guess that's why they fly into so many light bulbs. Nice post. A moth brain...that's cool Exclamation

Frez
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Steve West



Joined: 10 Oct 2004
Posts: 545
Location: Tucson, AZ USA

PostPosted: Thu Dec 09, 2004 8:51 pm    Post subject: Reply with quote

Hi Frez,

Glad you like the moth brain Cool

The stitching was a pain since there were so many threads of air tubes, the stitcher got all confused. I had to put manual control points in each frame connecting to the adjacent frames. If I recall, the stitcher worked about 2 hours working out the distortions, color blending, etc Evil or Very Mad

I originally tried a single image with the Nikon 4x plan, but I couldn't produce good illumination over the 4mm area. Besides, the resolution was too low to see anything cool. So a 20x stitch was called for.

Steve
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Frez



Joined: 08 May 2004
Posts: 419
Location: New Hampshire USA

PostPosted: Thu Dec 09, 2004 8:59 pm    Post subject: Reply with quote

You definately have something to be proud of for the fruits of your labor. Make sure to back up this file.
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twebster
Site Admin


Joined: 19 Apr 2004
Posts: 1518
Location: Phoenix "Valley of the Sun", Arizona, USA

PostPosted: Thu Dec 16, 2004 11:16 pm    Post subject: Reply with quote

Hi ya' Steve Very Happy

Thank you so much for sharing this with us. What a wonderful image and such seamless stitching. That's what makes it our Administrator's Appreciation image for the months of December/January. Great job, Steve Exclamation

All the best, my friend Very Happy
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Tom Webster
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Phoenix "The Valley of the Sun", Arizona, USA

Think about this...maybe Murphy is an optimist!!!
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Ken Ramos
Site Admin


Joined: 04 May 2004
Posts: 4809
Location: Western North Carolina

PostPosted: Fri Dec 17, 2004 4:02 am    Post subject: Reply with quote

Very Happy Congrats Steve, that is a great shot and some excellent work. I have never tried "stitching" images Cool .
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Kenneth Ramos
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Kens Microscopy
Reposts of my images within the galleries are welcome, as are constructive critical critiques.
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Steve West



Joined: 10 Oct 2004
Posts: 545
Location: Tucson, AZ USA

PostPosted: Sun Jan 09, 2005 9:46 pm    Post subject: Reply with quote

Hi all,

I'm very flattered that one of my pics was picked!

The shirt is wonderful and is of very high quality--thanks for sending it to me Mike!

Steve
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Kenv



Joined: 24 May 2004
Posts: 852
Location: New Zealand

PostPosted: Sun Jan 09, 2005 11:39 pm    Post subject: Reply with quote

Well done Steve.
Kenv
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