... or why not use infinity corrected objectives on a 160mm tube.
I equipped my Zeiss microscope with a phase condenser. I don't have any phase objective yet, so I tried my old 20x/0.50 American Optical infinity corrected objective, which happened to have a perfectly matching diameter of phase annulus. Results are rather disappointing. This is the best picture I could get. You can see contractile vacoules and a nucleus, but overall quality is poor. Resolution suffers a lot, the picture is blurry.
Anyone has a spare phase neofluar to sell?...
Poor man's phase contrast...
Moderators: MacroMike, nzmacro, Ken Ramos, twebster, S. Alden
Re: Poor man's phase contrast...
That's actually quite amazing Piotr, there seems to be quite a difference in how infinity objectives behave. How is this one doing in BF? I've tried once some infinity Zeiss (10 and 40x neofluar) on a Leitz 160mm stand, and couldn't really see a difference by quick glance, certainly not as dramatic as your image shows.piotr wrote:... or why not use infinity corrected objectives on a 160mm tube.
I equipped my Zeiss microscope with a phase condenser. I don't have any phase objective yet, so I tried my old 20x/0.50 American Optical infinity corrected objective, which happened to have a perfectly matching diameter of phase annulus. Results are rather disappointing. This is the best picture I could get. You can see contractile vacoules and a nucleus, but overall quality is poor. Resolution suffers a lot, the picture is blurry.
Anyone has a spare phase neofluar to sell?...
I'm having myself a Wild phase fluotar series, the 20/0.60 is an absolute stunner. You would probably have to make a condenser annulus yourslf, but it's well worth it!
Rene.
Rene, thank you for the comments.
The difference in brightfield is not as serious. The image is just a little bit more blurry and it seems to have an eyestraining effect. The phase contrast image looks a little bit better in real than on this picture, but not as good as on a dedicated AO scope.
I think I will experiment more. My poor imaging skills might be another source of the problem. And I hope to get a Wild objective soon!
The difference in brightfield is not as serious. The image is just a little bit more blurry and it seems to have an eyestraining effect. The phase contrast image looks a little bit better in real than on this picture, but not as good as on a dedicated AO scope.
I think I will experiment more. My poor imaging skills might be another source of the problem. And I hope to get a Wild objective soon!
Piotr