Hello all,
I wonder if anyone has tried using the 546nm GIF to attain maximum contrast with phase? IIUC (If I understand correctly) this was the specific frequency that phase optics are designed around.
All the best,
Mike
Use of 546nm Green Interference Filter with Phase
Moderators: MacroMike, nzmacro, Ken Ramos, twebster, S. Alden
Green interference filters $2.50 apiece
Yes!... and they improve things a bit, at least with my phase achromat objectives.
This picture I took using the green interference filter:
AO 45x/0.66 NA, phase contrast with green interference filter, Canon S100. Cheek epithelial cells.
By the way. Surplus Shed has them in stock, $2.50 apiece, 50x50 mm square. I'm not sure if they are exactly 546 nm, but close enough for me.
This picture I took using the green interference filter:
AO 45x/0.66 NA, phase contrast with green interference filter, Canon S100. Cheek epithelial cells.
By the way. Surplus Shed has them in stock, $2.50 apiece, 50x50 mm square. I'm not sure if they are exactly 546 nm, but close enough for me.
Piotr
Hello Piotr & Kenv:
Thanks for the confirmation re resolution improvement.
I have sourced a GIF with 512nm cut-on and 557 cut-off so that should be pretty close.
Piotr - thanks for the link to Surplus Shed - they always seem to have the odd ball items!
Kenv - yes, the green cast is 'unusual' but as you say, conversion to B&W takes care of that.
All the best,
Mike
Thanks for the confirmation re resolution improvement.
I have sourced a GIF with 512nm cut-on and 557 cut-off so that should be pretty close.
Piotr - thanks for the link to Surplus Shed - they always seem to have the odd ball items!
Kenv - yes, the green cast is 'unusual' but as you say, conversion to B&W takes care of that.
All the best,
Mike