Search found 25 matches

by Thomas Ashcraft
Sat Jun 10, 2006 6:25 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Proper darkfield microscopy?
Replies: 7
Views: 23631

Steve wrote: "DF is not everything-- If you are wanting to look at pond water for tiny beasties, Phase is very, very useful." Steve and all, Can you do decent if not optimum darkfield then going through phase contrast objectives with a darkfield stop in the condenser (and Koehler illumination)? I as...
by Thomas Ashcraft
Fri Jun 09, 2006 3:21 pm
Forum: Macro and Micro Technique and Technical Discussions
Topic: Proper darkfield microscopy?
Replies: 7
Views: 23631

Answering my own questions : I just discovered some excellent darkfield setup tutorials and primers at:

http://micro.magnet.fsu.edu/primer/tech ... field.html

http://micro.magnet.fsu.edu/primer/tech ... setup.html

:)
by Thomas Ashcraft
Fri Jun 09, 2006 2:06 pm
Forum: Macro and Micro Technique and Technical Discussions
Topic: Proper darkfield microscopy?
Replies: 7
Views: 23631

Proper darkfield microscopy?

First, thank you all for your patience in answering my questions. These are questions that I haven't found the answers to on the web after much digging and googling. So then, what are the best objectives for darkfield imaging? Brightfield objectives stopped down? Or phase contrast objectives stopped...
by Thomas Ashcraft
Thu Jun 08, 2006 4:56 pm
Forum: Macro and Micro Technique and Technical Discussions
Topic: Phase contrast microscope for brightfield too ?
Replies: 8
Views: 26195

"Since these new ( BF semi-plan phase ) Meiji objectives have more glass elements, they tend to transmit less light. We recommend for the best results and the brightest image that they be used only on the ML5000 series with the external 50 watt illuminator." I smell a rat . . . or at least a proble...
by Thomas Ashcraft
Thu Jun 08, 2006 8:37 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Phase contrast microscope for brightfield too ?
Replies: 8
Views: 26195

Charles and Steve, Maybe illumination power could also be a factor. In my studies since posting the original question I read on a Meiji microscope dealer's site that: "Since these new ( BF semi-plan phase ) Meiji objectives have more glass elements, they tend to transmit less light. We recommend for...
by Thomas Ashcraft
Wed Jun 07, 2006 7:21 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: Phase contrast microscope for brightfield too ?
Replies: 8
Views: 26195

Phase contrast microscope for brightfield too ?

Can phase contrast objectives also be used for quality brightfield viewing and photography? Or will you lose appreciable image quality if you use phase contrast objectives for brightfield imaging?

Thanks for any info in advance.

Thomas A
by Thomas Ashcraft
Wed Jun 07, 2006 6:45 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: What microscope for movies like these? - ( new member)
Replies: 6
Views: 21711

Ken wrote: "...... I was thinking of upgrading to an Axiophot. That microscope alone with only the basics is around $4000.00 or better! :roll: So I would imagine the Axioplan is much more, not counting the high NA objectives and other bells and whistles required." Hi Ken, That's serious money! My po...
by Thomas Ashcraft
Wed Jun 07, 2006 5:55 am
Forum: Macro and Micro Technique and Technical Discussions
Topic: What microscope for movies like these? - ( new member)
Replies: 6
Views: 21711

"If you don't mind used equipment, you can get some great deals on ebay - go to the business and industrial section and seach for a manufacturer's name such as Olympus, Nikon, Zeiss, Leica, Wild, American Optical, etc. Then start digging. I bought an Olympus Vanox research microscope with DIC for a ...
by Thomas Ashcraft
Sat Jun 03, 2006 7:32 pm
Forum: Macro and Micro Technique and Technical Discussions
Topic: What microscope for movies like these? - ( new member)
Replies: 6
Views: 21711

Hi Charles, Thanks for that detailed reply and for generously digging out those references. I have spent the time since your reply at "Nikon Microscopy U" on the web learning as much as possible. ( That's a pretty good resource!) Indeed, Stanford has enviable resources for sure. Yet it's also nice t...
by Thomas Ashcraft
Fri Jun 02, 2006 3:36 pm
Forum: Macro and Micro Technique and Technical Discussions
Topic: What microscope for movies like these? - ( new member)
Replies: 6
Views: 21711

What microscope for movies like these? - ( new member)

Greetings to all of you who think small like I do. My name is Thomas Ashcraft and I am a new member living in New Mexico, USA. I am in the process of manifesting the right microscope for videos and photography and could use a little advice as I am in the steep part of the learning curve at present a...